Rat Oligodendrocyte Nucleofector Kit
| Cat. No. | VPG-1009 |
| Optimized Protocol |  Download (PDF, 158 KB) |
| Transfection Efficiency | After 48 hours: 44±4% |
| Cell Database |  Oligodendrocyte, rat |
During multiple sclerosis, oligodendrocytes as well as the myelin sheath, which insulates axons, are destroyed. The Nucleofector technology is the first technology which permits an efficient non-viral gene transfer into these cells. This provides further insights into oligodendrocyte biology and dysfunction.
- Up to 50% transfection efficiency
- Maintenance of functionality
Nucleofection of rat oligodendrocytes.
Rat oligodendrocyte precursor cells were transfected using the rat Oligodendrocyte Nucleofector Kit and 5 µg of a plasmid encoding the enhanced green fluorescent protein eGFP. Cells were analyzed by fluorescence microscopy 48 hours post nucleofection.
(Photograph courtesy of Dr.W.Deng, Children's Hospital, Boston, MA, USA)
Components of Rat Oligodendrocyte Nucleofector Kit
Cat. No. VPG-1009, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Rat Oligodendrocyte Nucleofector Solution |
