Rat Neuron Nucleofector Kit
| Cat. No. | VPG-1003 |
| Optimized Protocol |  Download (PDF, 197 KB) |
| Transfection Efficiency | After 48 hours: 41±7.5% for dorsal root ganglion |
| Cell Database |  Dorsal root gang. (DRG), rat |
Rat neurons are frequently used as an experimental model to study neural cell function and to develop new therapies for neurodegenerative diseases. The Nucleofector technology offers the first non-viral method for efficient gene transfer into primary rat neurons.
- Transfection efficiencies of up to 45%
- Transgene expression for more than one week
- Maintenance of morphological and functional properties
- One Nucleofector kit for dorsal root ganglia as well as hippocampal or cortical neurons
Transfection efficiency of rat dorsal root ganglion neurons.
Primary dorsal root ganglion neurons were nucleofected with program G-13 and 3 μg of a plasmid encoding the enhanced green fluorescent protein eGFP. 48 hours post nucleofection, the cells were analyzed by fluorescence microscopy.
(Courtesy of Dr. B.D. Grubb, Dept. of Cell Physiology and Pharmacology, University of Leicester, UK)
Nucleofection of rat dorsal root ganglion neurons with eGFP cDNA.
Primary dorsal root ganglion neurons were nucleofected using the Rat Neuron Nucleofector Kit, program O-03 and a plasmid encoding enhanced green fluorescent protein eGFP. 48 hours post nucleofection, the cells were analyzed by fluorescence microscopy (A and B).
(Photograph courtesy of Dr. B.D. Grubb, Dept. of Cell Physiology and Pharmacology, University of Leicester, UK)
Components of Rat Neuron Nucleofector Kit
Cat. No. VPG-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Rat Neuron Nucleofector Solution |
