Mouse NSC (Neural Stem Cell) Nucleofector Kit
| Cat. No. | VPG-1004 |
| Optimized Protocol |  Download (PDF, 134 KB) |
| Transfection Efficiency | After 24 hours: 40±5% After 48 hours: 60±10% |
| Cell Database |  Neural stem cell (NSC), mouse |
NSC from mouse brain can be differentiated into various neural and glial cell types, potentially in the future this may contribute to the development of therapies for CNS disorders via replacement of damaged neural tissue. The Nucleofector technology presents a powerful tool to gain further insights into how these cells function.
- Up to 70% transfection efficiency
- Up to 90% viability
- Transgene expression for several days
- Differentiation into neurons and astrocytes possible
Nucleofection of mouse NSCs
Mouse neural stem cells were isolated from spinal cords of mouse embryos (E14-15) and cultured for 3 passages in medium containing EGF and FGF. Cells were transfected by nucleofection using program A33 and 3 µg of a plasmid encoding the enhanced green fluorescent protein eGFP. After nucleofection the cells were cultured in medium containing 1% FCS to initiate differentiation into neurons and astrocytes. 72 hours post nucleofection cells were analyzed by fluorescence microscopy.
(Courtesy of I. Richard and U. Bartsch, Center for Molecular Neurobiology, Hamburg, Germany)
Components of Mouse NSC (Mouse Neural Stem Cell) Nucleofector Kit
Cat. No. VPG-1004, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Mouse NSC Nucleofector Solution |
