Mouse ES Cell 96-well Nucleofector Kit
| Cat. No. | VHPH-1001 |
| Number of Reactions | 1x96 |
| Cat. No. | VHPH-2001 |
| Number of Reactions | 10x96 |
| Optimized Protocol |  Download (PDF, 0.7 MB) |
| Transfection Efficiency | After 24 hours: 86±4% for ES-D3% After 24 hours: 90±5% for ES-E14TG2a |
| Cell Database |  Embryonic stem (ES) cell, mouse |
Mouse ES cells are often used to study the biological basics of cell differentiation, tissue formation and embryo development. In addition transfected Mouse ES cells are used to generate knock-out mice.
- Transfection efficiencies up to 90%
- Viability of up to 80% after 24h
- Preservation of cell functionality (ability to differentiate)
Efficiency and viability of the mouse ES 96-well Nucleofector Kit
5 x 104 cells were nucleofected with program 96-CG-104 using 0.4 µg pmaxGFP, 4x104 cells were seeded in one well of a 96-well plate. Cells were analyzed 24 hours post nucleofection for GFP expression and viability using a Becton Dickinson FACSCalibur with HTS option. Cell viability is given in percent compared to non nucleofected control.
Well-to-well uniformity of reporter gene expression after 96-well nucleofection.
5 x 104 mouse ES cells were nucleofected with 0.4 µg of pmaxGFP using the Mouse ES Cell 96-well Nucleofector Kit. GFP activity was measured 24 hours post nucleofection (n=72, SD = ± 0.8% from mean). Wells without GFP expression are negative controls (cells in the 96-well Nucleofector Solution and plasmid DNA but without nucleofection)
Components of Mouse ES Cell 96-well Nucleofector Kit
Cat. No. VHPH-1001, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 0.45 | ml | Supplement |
| 2.025 | ml | Mouse ES Cell 96-well Nucleofector Solution |
Components
Cat. No. VHPH-2001, 10x96 Reactions
| 10 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 4.5 | ml | Supplement |
| 20.25 | ml | Mouse ES Cell 96-well Nucleofector Solution |
