Mouse Astrocyte Nucleofector Kit
| Cat. No. | VPG-1006 |
| Optimized Protocol |  Download (PDF, 131 KB) |
| Transfection Efficiency | After 48 hours: 60±10% |
| Cell Database |  Astrocyte, mouse |
Astrocytes play an important role in providing support to neurons and controlling the structural and functional elasticity of synapses. Furthermore, they may be involved in the pathogenesis of brain disorders. The Mouse Astrocyte Nucleofector Kit presents a powerful tool to gain further
insights into the biological role and function of these cells.
- Transfection efficiencies up to 60%
- Suitable for mouse astrocytes from cortex, midbrain or striatum
- Maintenance of functionality
Nucleofection of mouse astrocytes
Primary mouse astrocytes isolated from mouse embryos (E14) were transfected using the Mouse Astrocyte Nucleofector Kit and a plasmid encoding enhanced green fluorescent protein eGFP. 2 days post nucleofection, the cells were analyzed by fluorescence microscopy.
(Courtesy of Dr. S. Franken, Dr. M. Eckhardt, Prof. V. Gieselmann, Institute for Physiological Chemistry, University of Bonn)
Components of Mouse Astrocyte Nucleofector Kit
Cat. No. VPG-1006, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Mouse Astrocyte Nucleofector Solution |
