Chicken Neuron Nucleofector Kit
| Cat. No. | VPG-1002 |
| Optimized Protocol |  Download (PDF, 108 KB) |
| Transfection Efficiency | After 24 hours: 43±14% for hippocampal or cortical |
| Cell Database |  Neuron, hippocampal, chicken |
Chicken neurons are used as a model system to elucidate neurological disorders, such as Parkinson's disease. The Nucleofector technology is providing a new insight that will enable you to learn more about neurodegenerative diseases using this in vitro model system.
- Transfection efficiencies of more than 40%
- Transgene expression for more than one week
- Maintenance of morphological and functional properties
- One Nucleofector Kit for hippocampal neurons and dorsal root ganglia
Transfection efficiency of chicken hippocampal neurons.
Primary chicken hippocampal neurons were transfected by nucleofection with program G-13/G-013 and 3 μg of a plasmid encoding the enhanced green fluorescent protein eGFP. 24 hours post nucleofection, the cells were analyzed by fluorescence microscopy.
Nucleofection of embryonic chicken neurons.
Mixed glial cells isolated from chicken embryos (E7) were transfected using the Chicken Neuron Nucleofector Kit and a plasmid encoding the enhanced green fluorescent protein eGFP. Five days post nucleofection cells were analyzed by fluorescence microscopy showing neurite and axonal outgrowth.
Components of Chicken Neuron Nucleofector Kit
Cat. No. VPG-1002, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Chicken Neuron Nucleofector Solution |
