Chicken Neuron Nucleofector Kit
| Cat. No. | VPG-1002 |
| Optimized Protocol |  Download (PDF, 130 KB) |
| Transfection Efficiency | After 48 hours: 30±10% for dorsal root ganglion |
| Cell Database |  Dorsal root gang. (DRG), chicken |
Chicken neurons are used as a model system to elucidate neurological disorders, such as Parkinson's disease. The Nucleofector technology is providing a new insight that will enable you to learn more about neurodegenerative diseases using this in vitro model system.
- Transfection efficiencies of more than 30%
- Transgene expression for more than one week
- Maintenance of morphological and functional properties
- One Nucleofector Kit for dorsal root ganglia and hippocampal neurons
Transfection efficiency of chicken dorsal root ganglion neurons.
Primary chicken dorsal root ganglion neurons were nucleofected with program O-03 and 3 μg of a plasmid encoding the enhanced green fluorescent protein eGFP. 48 hours post nucleofection, the cells were analyzed by fluorescence microscopy.
(Courtesy of Dr. B. Eickholt, King´s College, London, UK).
Nucleofection of chicken dorsal root ganglion neurons with eGFP cDNA.
Primary dorsal root ganglion neurons were nucleofected using the Chicken Neuron Nucleofector Kit, program O-03 and a plasmid encoding enhanced green fluorescent protein eGFP. 48 hours post nucleofection, the cells were analyzed by fluorescence microscopy.
(Photograph courtesy of Dr. B. Eickholt, King´s College, London, UK.)
Components of Chicken Neuron Nucleofector Kit
Cat. No. VPG-1002, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Chicken Neuron Nucleofector Solution |
