Basic Neuron SCN Nucleofector Kit
| Cat. No. | VSPI-1003 |
| Optimized Protocol |  Download (PDF, 0.7 MB) |
Just Transfect as Many Neurons as You Need for One Coverslip!
amaxa's new Basic Neuron Small Cell Number (SCN) Nucleofector Kit makes high efficiency transfection of mammalian primary neurons possible for extremely low cell numbers. Now you can transfect just the number of cells needed for later culture and analysis (e.g. microscopic analyses of neuron growth and morphology). Benefit from a drastic reduction in both number of donor animals and amount of preparation time. The new, optimized SCN Nucleofector Solution, SCN cuvettes and programs make transfection efficiencies of up to 60% possible with down to 15,000 cells per nucleofection.
- Transfection of down to 15,000 cells per nucleofection - More experiments with fewer donor animals
- Excellent non-viral transfection efficiencies and viabilities – 50% and higher even for very difficult-to-transfect neurons like DRGs
SCN Nucleofection of freshly isolated mouse Dorsal Root Ganglion (DRG) cells.
15,000 DRGs (E15.5) were transfected with SCN Basic Neuro Program 6 and 0.4 µg pmaxGFP (green, B) and seeded on a coated coverslip. 24 hours post-nucleofection, cells were fixed and immunostained for beta-tubulin III (red, neuronal marker, A). A merge of both colours is shown in C. In this experiment, transfection efficiency as determined by fluorescence microscopy was approximated 60%. Data by courtesy of B. Eickholt, MRC Centre for Developmental Neurobiology, King‘s College, London, United Kingdom.
Components of Basic Neuron SCN Nucleofector Kit
Cat. No. VSPI-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified SCN cuvettes | |
| 10 | µg | pmaxGFP (for 25 reactions) |
| 0.1 | ml | SCN Supplement |
| 0.45 | ml | SCN Nucleofector Solution |
