Nucleofection of C6 glioma cells
| Cat. No. | VCA-1003 |
| Optimized Protocol |  Download (PDF, 0.8 MB) |
| Transfection Efficiency | After 5 hours: 70±6% for high cell survival (program U-09) After 24 hours: 87±1% for high cell survival (program U-09) After 5 hours: 91±2% for high transfection efficiency (program U-30) After 24 hours: 95±3% for high transfection efficiency (program U-30) |
| Cell Database |  C6 (ATCC) |
| Cell Clone |  ATCC CCL-107 |
The C6 glioma cell line is a widely used cell line in neurobiological research. Compared to primary neural cells, the C6 cell line offers some major advantages like easy accessibility and culture as well as a good availability of high cell numbers.
Nevertheless, the biggest disadvantage of the C6 cells is that they are difficult-to-transfect with commonly used non-viral transfection methods. Now, the Nucleofector technology simplifies the transfection of C6 cells. Transfection efficiencies of over 90% can be easily achieved with excellent cell viabilities of 75-80%.
Nucleofection of C6 glioma cells.
C6 cells were transfected using the Cell Line Nucleofector Kit V and a plasmid encoding the enhanced green fluorescent protein eGFP. 5 hours post nucleofection, cells were analyzed by light (top) and fluorescence microscopy (bottom).
Components of Cell Line Nucleofector Kit V
Cat. No. VCA-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution V |
