Mouse T Cell Nucleofector Kit

Cat. No.	VPA-1006
Optimized Protocol	Download (PDF, 231 KB)
Transfection Efficiency	After 24 hours: 37±4% for CD4+ After 24 hours: 39±7% for CD8+
Cell Database	T cell, mouse - BALB/c

Optimized Protocol	Download (PDF, 231 KB)
Transfection Efficiency	After 24 hours: 27±6% for CD4+ After 24 hours: 23±5% for CD8+
Cell Database	T cell, mouse - C57BL/6

Transfection of primary mouse T cells represents a major breakthrough in addressing research areas such as T cell function, activation, regulation and signaling in this model in vitro system. The combination of specific Nucleofector solution, electrical parameters and optimized recovery medium is
essential for transfection efficiency and post transfection survival of nucleofected mouse T cells.

Transfection efficiencies up to 40%
Viability after transfection up to 55%
Evaluated for C57BL/6 and BALB/c strains
Maintenance of functionality (e.g. stimulation)
Complete solution – optimized recovery medium included

Average transfection efficiencies for primary mouse T cells from C57BL/6 mice (CD4, CD8).

Nucleofected and non-nucleofected mouse T cells can be stimulated equally well.
Primary C57BL/6 mouse T cells were transfected with 2.5 µg pmaxGFP™. 3h post nucleofection cells were stimulated with plate bound anti-CD3ε (5 µg/ml) and anti-CD28 (2 µg/ml). 48 and 72h post nucleofection, CD4⁺ cells were analyzed for CD25 surface expression. Figure shows proportion of CD25-expressing cells among living CD4⁺ T cells. (%CD25 expression in unstimulated samples ranged from 10-20%.)

Components of Mouse T Cell Nucleofector Kit

Cat. No. VPA-1006, 25 Reactions

25		Plastic pipettes
25		Certified cuvettes
10	µg	maxGFP (for 5 reactions)
0.5	ml	Supplement
2.25	ml	Mouse T Cell Nucleofector Solution
100	ml	Mouse T Cell Nucleofector Medium
1	ml	Medium Component A
1	ml	Medium Component B