Mouse Macrophage Nucleofector Kit
| Cat. No. | VPA-1009 |
| Optimized Protocol |  Download (PDF, 431 KB) |
| Transfection Efficiency | After 24 hours: 47±6% After 48 hours: 24±6% |
| Cell Database |  Macrophage, mouse - C57BL/6 |
| Optimized Protocol | Download (PDF, 431 KB) |
| Transfection Efficiency | After 24 hours: 45±3% After 48 hours: 34±6% |
| Cell Database | Macrophage, mouse - BALB/c |
Macrophages are frequently used in studies on basic immunology and cancer research. With the Mouse Macrophage Nucleofector Kit you can now address areas such as gene regulation, signaling pathways or identification of genes responsible for differentiation in the murine in vitro model system.
- Transfection efficiency up to 50%
- Evaluated for macrophages from C57BL/6 and BALB/c strains
- For transfection of resting bone marrow derived macrophages
- Maintenance of functionality (e.g. activation)
Average transfection efficiencies and viabilities (% 7-AAD negative) of mouse macrophages (C57BL/6 and BALB/c strains) transfected with pmaxGFP.
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Example for the nucleofection of mouse macrophages with pmaxGFP.
Primary mouse macrophages (isolated from C57BL/6 mice) were transfected by nucleofection with 2 µg pmaxGFP™ and analyzed by light (top) and fluorescence microscopy (bottom) at 40x magnification. Transfected macrophages reveal cytoplasmic extrusions important for phagocytic function of macrophages.
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Mouse macrophages can be stimulated after nucleofection
Primary mouse macrophages (C57BL/6) were transfected with 2 µg pmaxGFP™. 6 hours post nucleofection the culture medium was changed and cells were stimulated with LPS. 24 hours post nucleofection TNFα production was determined by ELISA. Values were normalized to number of living cells after transfection and are expressed as pg/ml TNFα per 1000 cells. In DNA transfected samples a similar but slighlty reduced TNFα production can be detected. In non-stimulated samples no TNFα was detected.
Components of Mouse Macrophage Nucleofector Kit
Cat. No. VPA-1009, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Mouse Macrophage Nucleofector Solution |
