Human Stem Cell (H9) 96-well Nucleofector Kit
| Cat. No. | VHPH-5003 |
| Number of Reactions | 1x96 |
| Cat. No. | VHPH-5203 |
| Number of Reactions | 10x96 |
| Optimized Protocol |  Download (PDF, 309 KB) |
| Transfection Efficiency | After 24 hours: 64±3% |
| Cell Database |  Embryonic stem (ES) cell, human |
The genetic manipulation of human stem cells was difficult so far due to their resistance to high efficiency transfection. With the release of Nucleofector Kits dedicated to transfect these cells, this bottleneck has been overcome.
Now, the new Human Stem Cell (H9) 96-well Nucleofector Kit allows high performance Nucleofection of the stem cell line H9 in a 96-well format. Thus enabling high throughput approches to elucidate various aspects of stem cell differentiaton.
- 64% transfection efficiency
- up to 98% cell viability
- excellent preservation of pluripotency
Example showing typical Nucleofection results of H9.
H9 cells were transfected with 0.4µg pmaxGFP using the HSC (H9) 96-well Nucleofector Kit. 24 hours post Nucleofection cells were analyzed by light (A) or fluorescence (B) microscopy (data by courtesy of Jennifer Moore, Rutgers University, Piscataway, USA).
Transfection efficiency of human stem cells (H9) cells 24 hours post Nucleofection
2 x 105 cells were transfected with program 96-CB-150 using 0.8 μg pmaxGFP. Cells were analyzed 24 hours post Nucleofection by FACS with HTS option. Cell viability was analyzed by PI staining. Cell counts were compared to non transfected control.
H9 cells preserve pluripotency post Nucleofection.
H9 cells transfected with pmaxGFP maintain their undifferentiated state. Analysis after 24 hours shows expression of GFP (green) as well as of the pluripotency markers SSEA4 (red) and Oct4 (purple). The blue signals refer to nuclear staining by DAPI. (Data kindly provided by Jennifer Moore, Rutgers University, Piscataway, USA).
Components of Human Stem Cell (H9) 96-well Nucleofector Kit
Cat. No. VHPH-5003, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 30 | µg | pmaxGFP |
| 0.45 | ml | Supplement |
| 2.025 | ml | Human Stem Cell (H9) 96-well Nucleofector Solution |
Components of Human Stem Cell (H9) 96-well Nucleofector Kit
Cat. No. VHPH-5203, 10x96 Reactions
| 10 | Certified 96-well Nucleocuvette plate(s) | |
| 30 | µg | pmaxGFP |
| 4.5 | ml | Supplement |
| 20.25 | ml | Human Stem Cell (H9) 96-well Nucleofector Solution |
