Human Natural Killer (NK) Cell Nucleofector Kit
| Cat. No. | VPA-1005 |
| Optimized Protocol |  Download (PDF, 163 KB) |
| Transfection Efficiency | After 24 hours: 16±3.5% |
| Cell Database |  Natural killer (NK), human |
Cancer and immunology research were frequently hampered due to the inability to transfect primary NK cells non-virally. With the Human NK Cell Nucleofector Kit, a major breakthrough was accomplished enabling primary cells to be transfected instead of cell lines. This kit has overcome the major obstacles of NK cell transfection and with efficiencies of up to 20% can provide a major advancement to your research.
- First non-viral transfection technology for primary NK cells
- Up to 20% transfection efficiency
- Viability post nucleofection 50-60%
Nucleofection of primary human NK cells
Primary human natural killer cells were transfected with a plasmid encoding the enhanced green fluorescent protein eGFP using the Human NK Cell Nucleofector Kit and program U-01. Cells were analyzed by flow cytometry 24 hours post nucleofection. eGFP expression in natural killer cells is shown after nucleofection without (A) and with plasmid DNA (B). (Courtesy of J. Sundbäck and K. Kärre, Karolinska Institutet, Microbiology and Tumor Biology Center, Stockholm, Sweden)
Components of Human Natural Killer Cell Nucleofector Kit
Cat. No. VPA-1005, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Human NK Cell Nucleofector Solution |
