Human B Cell 96-well Nucleofector Kit

Cat. No.	VHPA-1001
Number of Reactions	1x96

Cat. No.	VHPA-2001
Number of Reactions	10x96

Optimized Protocol	Download (PDF, 422 KB)
Transfection Efficiency	After 24 hours: 28±6%
Cell Database	B cell, human

Efficient transfection of human B cells is a pre-requisite to enable research in fields such as chemokine/antigen response or B cell activation. The standard Human B Cell Nucleofector Kit for 100 µl cuvettes has been successfully used for both DNA and RNA transfection and its applicability in many research areas has been demonstrated in various publications. With the new Human B Cell 96-well Nucleofector Kit it is now possible to perform nucleofection in 96-well format.

For unstimulated and stimulated B cells
Up to 35% transfection efficiency
80% fewer cells required compared to standard Nucleofector
One protocol for DNA, siRNA, RNA

Transfection efficiency of freshly isolated human B cells 24 hours post nucleofection.
Freshly isolated human B cells were nucleofected with program 96-EO-117 and 0.4 µg of pmaxGFP. 24 h post nucleofection cells were analyzed by FACS. Cell viability is usually around 70% (%PI negative B cells) after 24h.

Uniform reporter gene expression of 96-well nucleofected human B cells
Freshly isolated human B cells were nucleofected with 0.4 µg pmaxGFP using the Human B Cell 96-well Nucleofector Kit. 24 hours post nucleofection cells were analyzed by flow cytometry. Wells 1 and 96 are negative controls of cells in 96-well Nucleofector Solution and plasmid DNA, but without nucleofection.

Components of Human B Cell 96-well Nucleofector Kit

Cat. No. VHPA-1001, 1x96 Reactions

1		Certified 96-well Nucleocuvette plate(s)
45	µg	pmaxGFP (for up to 96 reactions)
0.45	ml	Supplement
2.025	ml	Human B Cell 96-well Nucleofector Solution

Components

Cat. No. VHPA-2001, 10x96 Reactions

10		Certified 96-well Nucleocuvette plate(s)
45	µg	pmaxGFP (for up to 96 reactions)
4.5	ml	Supplement
20.25	ml	Human B Cell 96-well Nucleofector Solution