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Nucleofection of WEHI-231

Cat. No. VCA-1005
Number of Reactions 25
Optimized Protocol > Download (PDF, 162 KB)
Transfection Efficiency After 24 hours: 77±11%
Cell Database > WEHI-231 (ATCC)
Cell Clone > ATCC CRL-1702

Example of nucleofection for WEHI-231 cells (ATCC).

WEHI-231 cells were transfected using the Cell Line Nucleofector Kit L, program C-05 or C-005 and 2 µg pmaxGFP. 24 hours post nucleofection, the cells were analyzed by light (A) and fluorescence microscopy (B).

Components of Cell Line Nucleofector Kit L

Cat. No. VCA-1005, 25 Reactions

 25    Plastic pipettes
 25    Certified cuvettes
 30  µg  pmaxGFP (for 15 reactions)
 0.5  ml  Supplement
 2.25  ml  Cell Line Nucleofector Solution L

   
Immunology / HematologyImmunology / Hematology
Cell Lines

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