Nucleofection of P815
| Cat. No. | VCA-1002 |
| Optimized Protocol |  Download (PDF, 112 KB) |
| Transfection Efficiency | After 24 hours: 62±5% |
| Cell Database |  P815 (ATCC) |
| Cell Clone |  ATCC TIB-64 |
Nucleofection of P815 cells.
P815 cells were transfected using the Cell Line Nucleofector Kit T, Program C-05 (for Nucleofector I) or C-005 (for Nucleofector II) and 2µg pmaxGFP. 24 hours post nucleofection, the cells were analyzed by light (A) and fluorescent microscopy (B).
Components of Cell Line Nucleofector Kit T
Cat. No. VCA-1002, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution T |
