Nucleofection of Jurkat
| Cat. No. | VCA-1003 |
| Optimized Protocol |  Download (PDF, 205 KB) |
| Transfection Efficiency | After 24 hours: 88±1% |
| Cell Database |  Jurkat (ATCC) |
| Cell Clone |  ATCC TIB 152 |
| Cat. No. | VCA-1003 |
| Optimized Protocol | Download (PDF, 188 KB) |
| Transfection Efficiency | After 24 hours: 74±5% |
| Cell Database | Jurkat (DSMZ) |
Jurkat cells are one of the most frequently used blood cell lines within the immunology/hematology research field. With the Nucleofector technology gene transfer into these cells can easily and quickly be performed and optimal performance is guaranted.
Different Jurkat subclones derived from different sources may have divergent features and display an extremely high genetic instability. Due to this variety between different Jurkat clones optimal nucleofection conditions may vary for each clone. We strongly recommend the use of the Jurkat clones E6-1 (ATCC) or ACC 282 (DSMZ).
For nucleofection of other Jurkat clones, the optimal nucleofection conditions can easily be determined using the Cell Line Optimization Nucleofector Kit or alternatively please contact our Scientific Support Team for further information.
Gene transfer efficiencies and viability of Jurkat cells
Nucleofection of E6-1 (ATCC) or ACC 282 (DSMZ) Jurkat cells were nucleofected using the Cell Line Nucleofector Kit V, program X-01 (for Nucleofector I) or X-001 (for Nucleofector II) and 2µg pmaxGFP. 24 hours post nucleofection the cells were analyzed by FACS analysis for maxGFP expression. Cell viability was determined as PI negative cells.
Components of Cell Line Nucleofector Kit V
Cat. No. VCA-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution V |
