96-well nucleofection of Jurkat
| Cat. No. | VHCA-1001 |
| Number of Reactions | 1x96 |
| Cat. No. | VHCA-2001 |
| Number of Reactions | 10x96 |
| Optimized Protocol |  Download (PDF, 196 KB) |
| Transfection Efficiency | After 24 hours: 71±6% |
| Cell Database |  Jurkat (ATCC) |
| Cell Clone |  ATCC TIB 152 |
Transfection efficiency of 96-well nucleofected Jurkat, Clone E6-1 (ATCC) 24 hours post nucleofection.
Jurkat, Clone E6-1 (ATCC) were nucleofected with program 96-CL-120 and 1 µg of pmaxGFP. 24 h post nucleofection cells were analyzed by flow cytometry. Cell viability is usually around 80% (%PI negative cells) after 24h.
Uniform reporter gene expression of 96-well nucleofected Jurkat, Clone E6-1 (ATCC)
Jurkat, Clone E6-1 (ATCC) were nucleofected with a plasmid encoding firefly luciferase using the Cell Line Nucleofector Kit SE. 24 hours post nucleofection luciferase activity was measured. Wells without luciferase activity are negative controls of cells in Nucleofector Solution and plasmid DNA, but without nucleofection.
Components of Cell Line 96-well Nucleofector Kit SE
Cat. No. VHCA-1001, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 0.45 | ml | Supplement |
| 2.025 | ml | Cell Line 96-well Nucleofector Solution SE |
Components
Cat. No. VHCA-2001, 10x96 Reactions
| 10 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 4.5 | ml | Supplement |
| 20.25 | ml | Cell Line 96-well Nucleofector Solution SE |
