Jurkat Automation 96-well Nucleofector Kit
| Cat. No. | VHCA-4001 |
| Optimized Protocol |  Download (PDF, 0.7 MB) |
| Cell Database |  Jurkat (ATCC) |
| Cell Clone |  ATCC TIB 152 |
During an automated transfection process, cells have to be stored in Nucleofector Solution for a prolonged time, depending on the number of scheduled reactions. Depending on the duration of the pre-incubation step, the transfection efficiency and viability of the cells might change over time.
For Jurkat cells a special OP was developed, extending the possible storage time to 6 hours. The Jurkat Automation OP is based on a new Automation Kit that operates with 2 Nucleofector Solutions plus Supplement which are mixed prior to the experiment. The corresponding automation OP can only be used in combination with the Automation Kit. The Automation Kit can only be ordered as Customized Kit.
Efficiency and Viability after different incubation periods pre-Nucleofection on different positions of a Nucleocuvette plate (Column 1 + 5). Jurkat cells were nucleofected with program 96-CM-137 and 1 μg of pmaxGFP. 48 hours post nucleofection cells were analyzed on a Becton Dickinson FACSCalibur with HTS option. Cell viability was determined using PI-staining.
Components of Jurkat Automation 96-well Nucleofector Kit
Cat. No. VHCA-4001
| Certified 96-well Nucleocuvette plate(s) (*) | ||
| Supplement (*) | ||
| Cell Line 96-well Nucleofector Solution AA1 (*) | ||
| Cell Line 96-well Nucleofector Solution AA2 (*) | ||
| * (Plate numbers will be customized. Volume of the solutions is depending on the required overfill to cover void volumes etc.) |
