Rat Hepatocyte Nucleofector Kit
| Cat. No. | VPL-1003 |
| Optimized Protocol |  Download (PDF, 156 KB) |
| Transfection Efficiency | After 24 hours: 52±6% |
| Cell Database |  Hepatocyte, rat |
Rat hepatocytes are involved in numerous metabolic pathways and have important functions that include detoxification and synthesis of proteins, lipids and bile acids. With the first efficient non-viral transfection method for primary rat hepatocytes in hand you can now get a better understanding of new therapeutic agents and toxicity mechanisms. Transfection efficiencies of up to 55% can be reached using the Nucleofector Kit for freshly isolated rat hepatocytes, while cells remain functional.
- transfection efficiencies up to 55%
- viability up to 80%
- suitable for DNA and siRNA
- nucleofected hepatocytes remain functional
Nucleofection of primary rat hepatocytes.
Rat hepatocytes were transfected with pmaxGFP. 24 hours post nucleofection cells were analyzed by light (A) and fluorescence microscopy (B).Primary rat hepatocytes.
Nucleofected hepatocytes maintain their morphology and polarization.
Primary rat hepatocytes were transfected by nucleofection with pmaxGFP (A) or a plasmid containing the cDNA sequence for a plasma membrane receptor-YFP fusion protein (B). Cells were stained with antibodies against desmoplakin (A; blue) to visualize cell boundaries and against multidrug resistance protein 2 (MRP2; A+B; red) to show the apical, canalicular membrane. maxGFP was located in the cytosol of transfected cells (A). YFP fusion protein was correctly targeted
to both the basolateral and the apical membrane domain as shown by co-localization with MRP2 (B). These data prove normal formation of bile canaliculi in hepatocytes transfected by nucleofection.
N = nuclei; * = bile canaliculi.
(Data courtesy of V. Keitel, F. Schliess and D. Häussinger, Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University Düsseldorf, Germany)
Components of Rat Hepatocyte Nucleofector Kit
Cat. No. VPL-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Rat Hepatocyte Nucleofector Solution |
