Human Hepatocyte 96-well Nucleofector Kit
| Cat. No. | VHPL-1001 |
| Optimized Protocol |  Download (PDF, 264 KB) |
| Transfection Efficiency | After 24 hours: 54% |
| Cell Database |  Hepatocyte, human |
Human hepatocytes are frequently used to study metabolic pathways and toxic effects of new therapeutic agents. However, due to their low proliferation ability in-vitro, primary hepatocytes are difficult to transfect by classical, non-viral methods. Our new Nucleofector Kit solves this problem by offering excellent transfection rates for DNA and siRNA.
- Up to 54% transfection efficiency
- Up to 69% cell viability
- Cells maintain functional for up to 120 hours
Transfection efficiency of human hepatocytes 24 and 120 hours post nucleofection.
1 x 105 human hepatocytes were transfected with program 96-EX-114 using 1 µg pmaxGFP. Cells were seeded in one well of a 96-well plate. Cells were analyzed 24 and 120 hours post nucleofection by counting. Cell viability was analyzed by using the CellTiter Blue cell viability assay (Promega).
Example showing typical nucleofection results of human hepatocytes.
Cryopreserved human hepatocytes were transfected with 1 µg pmaxGFP using the Human Hepatocyte 96-well Nucleofector Kit. 120 hours post nucleofection cells were analyzed by light (Left) and fluorescence microscopy (Right).
Components of Human Hepatocyte 96-well Nucleofector Kit
Cat. No. VHPL-1001, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 30 | µg | pmaxGFP (for up to 96 reactions) |
| 0.45 | ml | Supplement |
| 2.025 | ml | Human Hepatocyte Nucleofector Solution |
