Nucleofection of HT-29
| Cat. No. | VCA-1001 |
| Optimized Protocol |  Download (PDF, 136 KB) |
| Transfection Efficiency | After 24 hours: 51±10% |
| Cell Database |  HT-29 (ATCC) |
| Cell Clone |  ATCC HTB-38 |
Example of nucleofection for HT-29 cells (ATCC).
HT-29 cells were transfected with the Nucleofector Kit R, program W-17 and a plasmid encoding the fluorescent protein, maxGFP. 24 hours post nucleofection the cells were analyzed by phase contrast (A) or fluorescence microscopy (B).
Components of Cell Line Nucleofector Kit R
Cat. No. VCA-1001, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution R |
