NHEM-Neo (Normal Human Epidermal Melanocyte-Neonatal) Nucleofector Kit
| Cat. No. | VPD-1003 |
| Optimized Protocol |  Download (PDF, 113 KB) |
| Transfection Efficiency | After 5 hours: 70±13% |
| Cell Database |  Melanocyte, (NHEM-neo), human (Cascade) |
Melanocytes, the pigment-producing cells of the epidermis, undergo malignant transformation in malignant melanoma. Melanoma cells can metastasize to almost every major organ and tissue.
Nucleofection of neonatal melanocytes is a major pre-requisite to elucidate this transformation process.
- Up to 80% transfection efficiency
- Reproducible non-viral transfection
- For DNA and siRNA transfection
Nucleofection of NHEM-Neo with eGFP cDNA.
NHEM-Neo were transfected using the NHEM-Neo Nucleofector Kit and a plasmid encoding enhanced green fluorescent protein eGFP. 24h post nucleofection cells were analyzed by light (A) and fluorescence microscopy (B).
Components of NHEM-Neo (Normal Human Epidermal Melanocyte-Neonatal) Nucleofector Kit
Cat. No. VPD-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | NHEM Nucleofector Solution |
