Human MSC (Mesenchymal Stem cell) Nucleofector Kit
| Cat. No. | VPE-1001 |
| Optimized Protocol |  Download (PDF, 132 KB) |
| Transfection Efficiency | After 24 hours: 47±12% |
| Cell Database |  Mesench. stem (MSC), human (Lonza) |
The ability of bone marrow derived MSC to differentiate into various cell types has generated enormous interest in many different research fields. For the first time an efficient non-viral transfection of these cells is possible through the use of the Human MSC Nucleofector Kit.
- Up to 60% transfection efficiency
- Maintenance of functional properties
Nucleofection of human mesenchymal stem cells.
Human MSC (Human Mesenchymal Stem Cells) were transfected using the Human MSC Nucleofector Kit, program U-23 and a plasmid encoding the fluoresent protein eGFP. 21 hours post nucleofection the cells were analyzed by flow cytometry (FACS). eGFP expression of Human MSC is shown after nucleofection without (A) and with plasmid DNA (B).
Microscopical image of transfected human mesenchymal stem cells.
Human MSC were transfected using the Human MSC Nucleofector Kit and a plasmid encoding the fluorescent protein eGFP. 24 hours post-nucleofection cells were analyzed by light (top) and fluorescence microscopy (bottom).
Components of Human MSC (Mesenchymal Stem Cell) Nucleofector Kit
Cat. No. VPE-1001, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Human MSC Nucleofector Solution |
