Human Keratinocyte Nucleofector Kit
| Cat. No. | VPD-1002 |
| Optimized Protocol |  Download (PDF, 155 KB) |
| Transfection Efficiency | After 24 hours: 36±6% for adult keratinocytes, darkly pigmented donor After 24 hours: 51±13% for adult keratinocytes, lightly pigmented donor |
| Cell Database |  Keratinocyte, adult, human (Cascade) |
| Optimized Protocol | Download (PDF, 143 KB) |
| Transfection Efficiency | After 24 hours: 53±13% for neonatal keratinocytes After 48 hours: 39±12% for neonatal keratinocytes |
| Cell Database | Keratinocyte, neonatal, human (Cascade) |
Transfection of primary keratinocytes is an essential tool for studying various cellular functions such as gene expression, intracellular signaling and promoter studies. Commonly used non-viral transfection methods usually display certain drawbacks such as low transfection efficiency and the induction of terminal differentiation. Nucleofection offers a new highly effective non-viral alternative that does not induce terminal differentiation in transfected keratinocytes (Distler et al. 2005).
- Up to 55% transfection efficiency
- For neonatal and adult keratinocytes
- Maintenance of functionality (e.g. no terminal differentiation)
- Cited for DNA and siRNA transfection
Nucleofection of primary human neonatal keratinocytes.
Human neonatal keratinocytes isolated from foreskin were transfected with a plasmid encoding the enhanced green fluorescent protein eGFP using the Human Keratinocyte Nucleofector Kit and program T-07. Cells were analyzed 24 hours post nucleofection by light (top) and fluorescence microcopy (bottom).
Nucleofection of primary human adult keratinocytes.
Primary human adult keratinocytes were transfected using the Human Keratinocyte Nucleofector Kit, program T-24 and 2.5 µg of a plasmid encoding enhanced green fluorescent protein eGFP. 24 hours post nucleofection, the cells were analyzed by light (top) and fluorescence microscopy (bottom).
Components of Human Keratinocyte Nucleofector Kit
Cat. No. VPD-1002, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Human Keratinocyte Nucleofector Solution |
