Nucleofection of A-375
| Cat. No. | VCA-1003 |
| Optimized Protocol |  Download (PDF, 227 KB) |
| Transfection Efficiency | After 24 hours: 72±2% |
| Cell Database |  A-375 (ATCC) |
| Cell Clone |  ATCC CRL-1619 |
Example of nucleofection for A-375 cells (ATCC).
A-375 cells were transfected using the Cell Line Nucleofector Kit V, program X-01 (for Nucleofector I) X-001 (for Nucleofector II) and 2 µg of pmaxGFP. 24 hours post-nucleofection, the cells were analyzed by light (A) and fluorescence (B) microscopy.
Components of Cell Line Nucleofector Kit V
Cat. No. VCA-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution V |
