Nucleofection of 3T3-L1 adipocytes

Introduction

Under appropriate incubation conditions, 3T3-L1 cells differentiate into an adipocyte phenotype exhibiting many of the morphological, biochemical, and insulin-responsive features of the normal rodent adipocyte. To date, most transfection experiments with expression vectors or siRNA in 3T3-L1 cells have been limited to the pre-adipocytes stage since they are easier to transfect using lipofection reagents. Using Nucleofection it is now possible to perform transfection experiments using both cell stages with superior efficiency and unimpaired viability.

Example of nucleofection for 3T3-L1 adipocytes (ATCC).

10-day differentiated 3T3-L1 adipocytes (ATCC) were nucleofected using the Cell Line Nucleofector Kit L, program A-33 (for Nucleofector I) / A-033 (for Nucleofector II) and 2 µg of pmaxGFP. 24 hours post nucleofection, the cells were analyzed by light (A) and fluorescence microscopy (B).