Rat Cardiomyocytes-Neonatal Nucleofector Kit
| Cat. No. | VPE-1002 |
| Optimized Protocol |  Download (PDF, 223 KB) |
| Transfection Efficiency | After 48 hours: 35±4% |
| Cell Database |  Cardiomyocyte, rat |
The efficient introduction of DNA and siRNA into cardiomyocytes is of great importance in the molecular characterization of cardiac muscle function and development. The lack of satisfactory non-viral transfection methods for cardiac cells, such as neonatal rat cardiomyocytes, was a major obstacle in cardiovascular research. With the Nucleofector technology you can now achieve efficient transfection and address areas such as cardiac gene regulation and differentiation and the characterization of functional cardiac proteins.
- Up to 35% transfection efficiency
- Ideal for neonatal rat cardiomyocytes
- First efficient non-viral transfection technology
- Includes detailed cell isolation protocol
Nucleofection of neonatal rat cardiomyocytes
Primary neonatal rat cardiomyocytes were transfected by nucleofection with a plasmid encoding DsRed. 2 days post nucleofection, the cells were analyzed by fluorescence microscopy. Fig A shows DsRed expressing cells. Cardiomyocytes stained with FITC-labeled tropomyosin (a fibrous protein that is part of the muscle fibers) antibody are shown in fig. B. Fig. C is an overlay.
(Photographs courtesy of F. Engel and M. Keating, Cardiology Department, Childrens' Hospital, Havard Medical School, Boston, MA, USA)
Components of Rat Cardiomyocyte-Neonatal Nucleofector Kit
Cat. No. VPE-1002, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Rat Cardiomyocyte Nucleofector Solution |
