HCAEC (Human Coronary Artery Endothelial Cell)
| Cat. No. | VPB-1001 |
| Optimized Protocol |  Download (PDF, 167 KB) |
| Transfection Efficiency | After 24 hours: 57±11% |
| Cell Database |  Endothelial, coronary art.(HCAEC), human (Lonza) |
The coronary endothelium is important for cardiac function and coronary blood flow. Formerly the efficient gene transfer into coronary endothelial cells was limited to viral systems. With nucleofection of coronary endothelial cells you can now address various fields in cardiovascular research such as thrombosis, atherosclerosis and hypertension.
- Up to 60% transfection efficiency
- Suitable for DNA and siRNA
Nucleofection of HCAEC.
HCAEC were transfected with a plasmid encoding the fluorescent protein eGFP using the HCAEC Nucleofector Kit. 25 hours post nucleofection cells were analysed by light (top) and fluorescence microscopy (bottom).
Components of HCAEC (Human Coronary Artery Endothelial Cell) Nucleofector Kit
Cat. No. VPB-1001, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | HCAEC Nucleofector Solution |
