MEF (Mouse embryonic fibroblast) Nucleofector Kits
| Cat. No. | VPD-1006 |
| MEF Starter Nucleofector Kit | |
| Optimized Protocol |  Download (PDF, 411 KB) |
| Transfection Efficiency | After 24 hours: 43±8% |
| Cell Database |  Embryonic fibroblast (MEF), mouse |
| Cat. No. | VPD-1004 |
| MEF Nucleofector Kit 1 | |
| Optimized Protocol | Download (PDF, 0.9 MB) |
| Transfection Efficiency | After 24 hours: 43±8% |
| Cat. No. | VPD-1005 |
| MEF Nucleofector Kit 2 | |
| Optimized Protocol | Download (PDF, 0.9 MB) |
| Transfection Efficiency | After 24 hours: 43±8% |
MEF display significant genotypic and phenotypic variations depending on the strain and the genetic background of the mice they were isolated from as well as the immortalization strategy used. Thus, it was impossible to transfect some MEF cell types as each could behave differently with respect to the optimal conditions for efficient transfection. amaxa has resolved this drawback by developing two MEF Nucleofector Kits (1 and 2) and an easy-to-use MEF Starter Nucleofector Kit.
- Fast and individual nucleofection of your MEF cells
- Two different MEF Kits (1 and 2) to address genotypic and phenotypic variations
- MEF Starter Kit for fast determination of ideal nucleofection conditions
- Up to 50% transfection efficiency after 24h
The MEF Starter Nucleofector Kit is designed to determine of the ideal nucleofection conditions for each MEF cell type. This is done in a total of just 4 reactions using MEF 1 and MEF 2 Nucleofector Solution with two different programs. In the next step the appropriate MEF Nucleofector Kit (1 or 2) can be used to perform the experiments.
Nucleofection of primary MEF
Spontaneously immortalized mouse embryonic fibroblasts (strain:C57B6Jx129SV) were transfected using the MEF Nucleofector Kit 1 and a plasmid encoding the enhanced green fluorescent protein eGFP. 24 hours post nucleofection, the cells were analyzed by fluorescence microscopy.
(Photograph courtesy of Dr. H. Hermanns and Prof. P.H. Heinrich, University of Aachen, Germany)
Components of MEF Starter Nucleofector Kit
Cat. No. VPD-1006, 10 Reactions
| 10 | Plastic pipettes | |
| 10 | Certified cuvettes | |
| 20 | µg | pmaxGFP (for 10 reactions) |
| 2x0.1 | ml | Supplement |
| 0.45 | ml | MEF 1 Nucleofector Solution |
| 0.45 | ml | MEF 2 Nucleofector Solution |
Components of MEF Nucleofector Kit 1
Cat. No. VPD-1004, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | MEF 1 Nucleofector Solution |
Components of MEF Nucleofector Kit 2
Cat. No. VPD-1005, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | MEF 2 Nucleofector Solution |
