Human Macrophage 96-well Nucleofector Kit

Cat. No.	VHPA-1008
Number of Reactions	1x96

Cat. No.	VHPA-2008
Number of Reactions	10x96

Optimized Protocol	Download (PDF, 230 KB)
Transfection Efficiency	After 24 hours: 42±2% for 1x10^5 cells
Cell Database	Macrophage, human

Human macrophages play a key role in cell-mediated immunity. Hence, studying their functionality and role in health and disease is of great interest for pharmacologists, immunologists and cancer research scientists. amaxa's R&D team developed a Human Macrophage 96-well Nucleofector Kit enabling scientists to conduct high throughput transfection in these cells.

Transfection efficiencies up to 42%
Viability of 60% after 24h
80% fewer cells required compared to standard Nucleofector

Transfection efficiency of human Macrophages
1 x 10⁵ cells were nucleofected with program 96-DP-148 using 0.4 µg pmaxGFP. Cells were analyzed 24 hours post nucleofection using a Becton Dickinson FACSCalibur with HTS option. Cell viability at 24 hours is approximately 60%

Well-to-well uniformity of SEAP reporter gene expression after 96-well nucleofection.
1 x 10⁵ cells were nucleofected with 0.4 µg of an expression vector encoding secreted alkaline phosphatase (SEAP) using the Human Macrophage 96-well Nucleofector Kit. SEAP enzyme activity was measured 24 hours post nucleofection (n= 36, SD = ± 14,17 from mean). Wells without SEAP enzyme activity are negative controls (cells in the 96-well Nucleofector Solution and plasmid DNA but without nucleofection).

Components of Human Macrophage 96-well Nucleofector Kit

Cat. No. VHPA-1008, 1x96 Reactions

1		Certified 96-well Nucleocuvette plate(s)
45	µg	pmaxGFP (for up to 96 reactions)
0.45	ml	Supplement
2.025	ml	Human Macrophage 96-well Nucleofector Solution

Components

Cat. No. VHPA-2008, 10x96 Reactions

10		Certified 96-well Nucleocuvette plate(s)
45	µg	pmaxGFP (for up to 96 reactions)
4.5	ml	Supplement
20.25	ml	Human Macrophage 96-well Nucleofector Solution