Nucleofection of NCI-H1299
| Cat. No. | VCA-1004 |
| Optimized Protocol |  Download (PDF, 257 KB) |
| Transfection Efficiency | After 24 hours: 99±0.2% |
| Cell Database |  NCI-H1299 [H1299] (ATCC) |
| Cell Clone |  ATCC CRL-5803 |
Example of nucleofection for NCI-H1299 cells (ATCC).
NCI-H1299 cells (ATCC) were transfected using the Cell Line Nucleofector Kit C, program X-05 or X-005 and 2 µg of pmaxGFP. 24 hours post nucleofection, the cells were analyzed by light (A) and fluorescence microscopy (B).
Components of Cell Line Nucleofector Kit C
Cat. No. VCA-1004, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution C |
