96-well nucleofection of MCF-7
| Cat. No. | VHCA-1001 |
| Number of Reactions | 1x96 |
| Cat. No. | VHCA-2001 |
| Number of Reactions | 10x96 |
| Optimized Protocol |  Download (PDF, 405 KB) |
| Transfection Efficiency | After 24 hours: 72±3% |
| Cell Database |  MCF7 (ATCC) |
| Cell Clone |  ATCC HTB-22 |
Transfection efficiency of 96-well nucleofected MCF7 (ATCC) 24 hours post nucleofection.
MCF7 (ATCC) were nucleofected with program 96-EN-130 and 0.4 µg of pmaxGFP. 24 h post nucleofection cells were analyzed by flow cytometry. Cell viability is usually around 60% (%PI negative cells) after 24h.
uniform reporter gene expression of 96-well nucleofected MCF7(ATCC)
MCF7 (ATCC) were nucleofected with pmaxGFP using the Cell Line 96-well Nucleofector Kit SE. 24 hours post nucleofection cells were analyzed by flow cytometry. Wells without GFP expression are negative controls of cells in Nucleofector Solution and plasmid DNA, but without nucleofection.
Components of Cell Line 96-well Nucleofector Kit SE
Cat. No. VHCA-1001, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 0.45 | ml | Supplement |
| 2.025 | ml | Cell Line 96-well Nucleofector Solution SE |
Components
Cat. No. VHCA-2001, 10x96 Reactions
| 10 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 4.5 | ml | Supplement |
| 20.25 | ml | Cell Line 96-well Nucleofector Solution SE |
