Nucleofection of HT-1080
| Cat. No. | VCA-1002 |
| Optimized Protocol |  Download (PDF, 151 KB) |
| Transfection Efficiency | After 24 hours: 74±14% |
| Cell Database |  HT-1080 (ATCC) |
| Cell Clone |  ATCC CCL-121 |
Example of nucleofection for HT-1080 cells (ATCC).
HT-1080 cells were transfected using the Cell Line Nucleofector Kit T, program L-05 or L-005 and 2 µg of pmaxGFP. 24 hours post-nucleofection, the cells were analyzed by light (A) and fluorescence (B) microscopy.
Components of Cell Line Nucleofector Kit T
Cat. No. VCA-1002, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution T |
