Nucleofection of HCT 116
| Cat. No. | VCA-1003 |
| Optimized Protocol |  Download (PDF, 145 KB) |
| Transfection Efficiency | After 24 hours: 78±4% |
| Cell Database |  HCT 116 (ATCC) |
| Cell Clone |  ATCC CCL-247 |
Example of nucleofection for HCT 116 cells (ATCC).
HCT 116 cells (ATCC) were transfected using the Cell Line Nucleofector Kit V and 2 µg of pmaxGFP. 24 hours post nucleofection, the cells were analyzed by light (top) and fluorescence microscopy (bottom).
Components of Cell Line Nucleofector Kit V
Cat. No. VCA-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution V |
