Nucleofection of Suspension CHO Cells
| Cat. No. | VCA-1003 |
| Optimized Protocol |  Download (PDF, 191 KB) |
| Transfection Efficiency | After 24 hours: 92±4% |
| Cell Database |  CHO [suspension] (ECACC) |
Average transfection efficiencies and viabilities (% PI-negative) of suspension CHO cells (ECACC).
Cells were transfected by nucleofection with program U-24 or U-024 and 2.5 µg of pmaxGFP. 24 hours post nucleofection, the cells were analyzed by flow cytometry.
Specific productivities (µUnits/cell/day) of hSEAP referred to a cell density of 106 cells/ml seeded after the respective transfection.
5x107 suspension CHO cells [ECACC] were transfected using either 290 µl LipofectamineTM 2000 (58 µg pDRIVE-hSEAP) or nucleofection (=5 samples; program U-24 or U-024; Cell Line Kit V; 25 µg pDRIVE-hSEAP in total). Post transfection cells were cultivated as batches in spinner flasks containing 50 ml culture medium for 5 days under non-regulated conditions.
Components of Cell Line Nucleofector Kit V
Cat. No. VCA-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution V |
