96-well Nucleofection of CHO-S
| Cat. No. | VHCA-1003 |
| Number of Reactions | 1x96 |
| Cat. No. | VHCA-2003 |
| Number of Reactions | 10x96 |
| Optimized Protocol |  Download (PDF, 190 KB) |
| Transfection Efficiency | After 24 hours: 84±6% for 2x10^5 cells After 24 hours: 86±6% for 2x10^6 cells |
| Cell Database |  CHO-S [suspension] (Invitrogen) |
Transfection efficiency of CHO-S cells 24 hours post nucleofection.
2 x 105 or 2 x 106 cells were nucleofected with program 96-FF-137 and 0.4 µg pmaxGFP. 24 hours post nucleofection cells were analyzed on a Becton Dickinson FACSCalibur with HTS option. Cell viability is usually around 50% for both cell numbers after 24 hours.
Well-to-well uniformity of SEAP reporter gene expression after 96-well nucleofection.
2 x 105 CHO-S cells were nucleofected with 0.4 µg of an expression vector encoding the SEAP gene using the Cell Line 96-well Nucleofector Kit SG. 24 hours post nucleofection SEAP enzyme activity was measured (n=80, SD = ± 6.1% from mean). Wells without SEAP enzyme activity are negative controls of cells in 96-well Nucleofector Solution and plasmid DNA but without nucleofection.
Components of Cell Line 96-well Nucleofector Kit SG
Cat. No. VHCA-1003, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 0.45 | ml | Supplement |
| 2.025 | ml | Cell Line 96-well Nucleofector Solution SG |
Components
Cat. No. VHCA-2003, 10x96 Reactions
| 10 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 4.5 | ml | Supplement |
| 20.25 | ml | Cell Line 96-well Nucleofector Solution SG |
