96-well nucleofection of 293
| Cat. No. | VHCA-1002 |
| Number of Reactions | 1x96 |
| Cat. No. | VHCA-2002 |
| Number of Reactions | 10x96 |
| Optimized Protocol |  Download (PDF, 162 KB) |
| Transfection Efficiency | After 24 hours: 83±9% |
| Cell Database |  293 (ATCC) |
| Cell Clone |  ATCC CRL-1573 |
Transfection efficiency of 96-well nucleofected 293 (ATCC) 24 hours post nucleofection.
293 (ATCC) were nucleofected with program 96-CM-130 and 0.4 µg of pmaxGFP. 24 h post nucleofection cells were analyzed by flow cytometry. Cell viability is usually around 90% (%PI negative cells) after 24h.
uniform reporter gene expression of 96-well nucleofected 293 (ATCC)
293 (ATCC) were nucleofected with pmaxGFP using the Cell Line Nucleofector Kit SF. 24 hours post nucleofection cells were analyzed by flow cytometry. Wells without GFP expression are negative controls of cells in Nucleofector Solution and plasmid DNA, but without nucleofection.
Components of Cell Line 96-well Nucleofector Kit SF
Cat. No. VHCA-1002, 1x96 Reactions
| 1 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 0.45 | ml | Supplement |
| 2.025 | ml | Cell Line 96-well Nucleofector Solution SF |
Components
Cat. No. VHCA-2002, 10x96 Reactions
| 10 | Certified 96-well Nucleocuvette plate(s) | |
| 45 | µg | pmaxGFP (for up to 96 reactions) |
| 4.5 | ml | Supplement |
| 20.25 | ml | Cell Line 96-well Nucleofector Solution SF |
