amaxa eNews #5 amaxa biosystems
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FAQs on Nucleofection in Cancer Research


Is it possible to transfect mRNA of tumor cells into antigen-presenting cells like B cells or dendritic cells with the Nucleofector Technology?
Can I use the protocol for MCF7 cells for other breast cancer cell lines?
Can I use nucleofection to create a stable knockdown in a cancer cell line?
What protocol should I use for nucleofection® of patient derived blood samples, e.g. leukemia or lymphoma cells?
Is it possible to transfect siRNA into blood cells from NHL patients?
I have great efficiency for my cancer cell line with pmax-GFP but lower results with my construct. Do you have any suggestions on how to improve the efficiency?
In your optimized protocols for MDA-MB-231 and MDA-MB-468, you say to culture the cells without CO2. Why?
For more FAQs browse our FAQ database.
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The Nucleofector Technology, comprising Nucleofection Process, Nucleofector Device, Nucleofector Solutions, Nucleofector 96-well Shuttle System and Nucleocuvette plates and modules is covered by patent and/or patent pending rights owned by amaxa AG.

amaxa, Nucleofector, nucleofection, maxGFP, 96-well Shuttle and Nucleocuvette are either registered trademarks or trademarks of amaxa AG in the U.S. and/or Germany and/or other countries. Other product and company names mentioned herein are the trademarks of their respective owners.

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