amaxa eNews #4 amaxa biosystems
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FAQs on Nucleofection in Neurobiology


I'm transfecting neurons in a mixed glial culture. Is there an 'optimal' way to influence cell survival and select neurons after nucleofection®?
What is the optimal age of the pups we should use for DRG preparation?
I have been having trouble with "clumping" with my PC12 cells. Do you have any recommendations?
What is a Neural Stem Cell (NSC)?
Do I need a pure neuronal culture for Nucleofection?
Do NSCs (Neural Stem Cells) differentiate in co-culture with astrocytes?
I routinely culture neural stem cells as neurospheres. Can these be transfected by nucleofection®?
Are there any effects on the differentiation of neural stem cells following nucleofection®?
In some amaxa related publications SATO medium is recommended for DRG cell culture. Do you have more information about this medium?
How many cortical neurons can I expect from a single pup?
For more FAQs browse our FAQ database.
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The Nucleofector Technology, comprising Nucleofection Process, Nucleofector Device, Nucleofector Solutions, Nucleofector 96-well Shuttle System and Nucleocuvette plates and modules is covered by patent and/or patent pending rights owned by amaxa AG.

amaxa, Nucleofector, nucleofection, maxGFP, 96-well Shuttle and Nucleocuvette are either registered trademarks or trademarks of amaxa AG in the U.S. and/or Germany and/or other countries. Other product and company names mentioned herein are the trademarks of their respective owners.

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