amaxa eNews #3 amaxa biosystems
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FAQs on Nucleofection and RNAi


Can I use the Nucleofector technology for RNAi applications? How do I start?
What is the amount of siRNA needed per reaction?
What are the best time points for analysis of my siRNA experiments?
Do you have any siRNA nucleofection results using concentrations lower than 50nM?
Do you have any helpful calculations or conversion information for siRNA?
Why am I not able to detect my fluorescent label (e.g. FITC) after 24 or 48 hours post-nucleofection?
Do you recommend a particular supplier for siRNA duplexes?
Is it possible to pre-plate substrates when using the 96-well Shuttle for nucleofection?
What kind of buffer do you recommend for resuspending my siRNA?
What is the sequence of the siRNA oligo supplied in your siRNA Test Kit?
For more FAQs browse our FAQ database.
       More Information

If your question is not listed please do not hesitate to contact our Scientific Support Team:
  Europe/World  USA
 
phone  +49(0)221-99199-400  (888) 632-9110
 
fax  +49(0)221-99199-499  (888) 632-9112
 
e-mail  scientific-support.nucleofection@lonza.com  scientific-support.nucleofectionUS@lonza.com 

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The Nucleofector Technology, comprising Nucleofection Process, Nucleofector Device, Nucleofector Solutions, Nucleofector 96-well Shuttle System and 96-well Nucleocuvette plates and modules is covered by patent and/or patent pending rights owned by amaxa AG.

amaxa, Nucleofector, nucleofection, maxGFP, 96-well Shuttle and Nucleocuvette are either registered trademarks or trademarks of amaxa AG in the U.S. and/or Germany and/or other countries. Other product and company names mentioned herein are the trademarks of their respective owners.

amaxa disclaims all warranties, whether expressed or implied, including any warranty as to the quality, accuracy, safety, or suitability of the information provided in this e-newsletter for any particular purpose. Any use of the information contained on any page of this e-newsletter is evidence of agreement with these terms of use.