Cell & Reagent Calculator for 96-well Shuttle

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GENERAL	Amount	Unit	Default
number of transfections		#	96
cells / transfection		#
void volume comp. 20µl + X %		%	20

SUBSTRATES
pmaxGFP (0.2µg/µl) / reaction		µg
pmaxGFP concentration (stock)		µg/µl
DNA / transfection		µg
DNA concentration (stock)		µg/µl
siRNA / transfection in µg		µg
siRNA concentration (stock)		µg/µl
siRNA / transfection in µM		µM
siRNA concentration (stock)		µM

SUBSTRATE VOLUMES
pmaxGFP (/well)		µl
pmaxGFP (total)		µl
DNA (/well)		µl
DNA (total)		µl
siRNA (µg-based, /well)		µl
siRNA (µg-based, total)		µl
siRNA (µM-based, /well)		µl
siRNA (µM-based, total)		µl

SOLUTIONS
Nucleofector Solution		µl
Supplement		µl
total volume		µl

CELLS
cell number in total volume		#

RINSING MEDIUM POST TRANSFECTION
medium / well post transfection		µl	80
min. vol. of prewarmed medium		ml

CELL CULTURE POST TRANSFECTION
transfer volume to cell culture		µl	25/50
total cell culture volume		µl
preplated medium volume / well		µl