New Potentials in Target Validation: RNAi Library Screening using difficult-to-transfect Cell Lines
A general trend towards the use of primary cells and challenging cell lines demands a highly efficient and reliable transfection technology. This is especially true for successful RNAi library screens (such as in target validation studies) where efficient transfection of cells with siRNA duplexes or shRNA vectors is essential. The Nucleofector technology provides unique access to primary cell and cell line based siRNA screens delivering data of outstanding quality.
siRNA-mediated depletion of vimentin-mRNA in Jurkat cells Jurkat E6-1 (ATCC TIB 152) (2x105) were transfected with a siRNA duplex directed against endogenous vimentin. 24h post transfection vimentin mRNA levels were analyzed by RealTime PCR. Relative expressions compared to untreated control sample (well 31, set to 100%) are shown. (External data kindly provided by C. Merz, Schering AG, Berlin)
