Opening New Horizons: Efficient 96-well Transfection of Primary Cells
Transfection methods such as lipofection or electroporation yield satisfactory results for standard cell lines only, whereas many cell lines and especially primary cells are difficult or even impossible to transfect with conventional methods. Using the Nucleofector 96-well Shuttle, primary cells can be reliably transfected with high efficiency and high viability in a high throughput format. Thereby, applications such as target validation can now for the first time be performed in primary cells, hence drastically increasing result reliability.
Benefits
High convenience
- Optimized protocols for each cell type with details on cell isolation and culture
Flexible cell numbers
- Between 104 and 106 cells in 20 µl transfection volume
Access to difficult-to-transfect cell types
- Neurons, T cells and other difficult-to-transfect cells types can be transfected
Short time-to-results
- Expression within hours – from transfection to analysis in one day
Example for the transfection of primary cells using the 96-well Shuttle. Cells were transfected with 400 ng pmaxGFP and analyzed for maxGFP expression on a 
Becton Dickinson FACSCalibur with HTS option, 24h post nucleofection. Cell viabilities range between 30% and 92% depending on the cell type.
Cell-type specific 96-well Nucleofector Kits
human T cells- mouse T cells
- human B cells
- Endothelial cells, e.g. HUVEC
- Fibroblasts, e.g. NHDF-neo
- Neurons, e.g. rat Cortical or Hippocampal Neurons
... more Primary Cell 96-well Nucleofector Kits can be found here.
