The Ideal Tool for Transfection of Cell Lines for Screening Applications
The Nucleofector technology can be used for transfection of virtually any type of cell line such as commonly-used cell lines or difficult-to-transfect cell lines such as suspension cells. High transfection efficiencies combined with high cell viability and the parallel 96-well approach make it the ideal tool for RNAi library screening.
Benefits
High efficiencies
- siRNA: up to 99% transfer efficiency
- DNA: up to 95% transfection efficiency
Fast and easy optimization
- Optimization of transfection conditions for virtually any cell line within one plate
High convenience
- Optimized protocols available for cell lines with details on cell isolation and culture
Access to difficult-to-transfect cell lines
- Transfection of suspension cell lines
Ideal for high throughput applications
- e.g. RNAi library screens
Highly efficient transfection of various cell lines using the 96-well Shuttle. Cell lines were transfected with 400 ng pmaxGFP and analyzed for maxGFP expression on a 
Becton Dickinson FACSCalibur with HTS option, 24h post nucleofection. Cell viabilities range between 50% and 97% depending on the cell line.
Optimized Protocols exist for
... more Cell Line protocols can be found 
here.
Cell Line Optimization Kit
- The Optimization Kit covers cell lines, for which an optimized protocol does not yet exist
