HiFect - Generation of Biologically Relevant Results

Transfected cells remain functional.
HEK293 cells were cotransfected with a plasmid containing the cDNA sequence for a beta-arrestin-GFP fusion protein and a plasmid containing the cDNA sequence for a somatostatin receptor using HiFect. 24 hours post transfection the beta-arrestin-GFP fusion protein was located in the cytosol of cells (A). 5 minutes after addition of somatostatin the beta-arrestin construct is targeted to the cell membrane to bind the activated somatostatin receptor (B). 40 min after addition of somatostatin the beta-arrestin-GFP fusion protein and the somatostatin receptor internalize together (C).
(Data courtesy of Stefan Schulz, Institute for Pharmacology and Toxicology, University of Magdeburg, Germany)

Transfected cells maintain their normal morphology.
HEK293 cells were transfected with pmaxGFP (A) or a plasmid containing the cDNA sequence for a plasma membrane receptor-YFP fusion protein using HiFect (B). Cells were stained with propidium iodide to visualize cell nuclei (A; red). maxGFP was located in the cytosol of transfected cells (A). YFP fusion protein was correctly targeted to the cell membrane (B). The overall transfection efficiency was 80%.
HepG2 cells were transfected with an YFP expression plasmid using HiFect (C). The overall transfection efficiency was 40%.
(Data courtesy of V. Keitel, F. Schliess and D. Häussinger, Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University Düsseldorf, Germany)