hTC Culture Medium for human T cells
| Cat. No. | VZB-1002 |
| Cell Database |  T cell, unstim., human |
| Size | 500 ml |
| Cat. No. | VZB-4002 |
| Optimized Protocol |  Download (PDF, 47 KB) |
| Cell Database | T cell, unstim., human |
| Size | 4 x 500 ml |
A major pre-requisite for successful research on primary human T cells is the optimal culture medium supporting high viabilities. The hTC Culture Medium is specially optimized for human T cells and can be used for both: general T cell culture or culture of transfected T cells.
- optimal nutritional environment for T lymphocyte culture
- specialized to maintain high cell viability of cultured T lymphocytes
- high viability and transfection efficiency of transfected T lymphocytes
- ideal for maintenance of functionality of transfected T lymphocytes (e.g. activation)
Optimal survival in hTC Culture Medium.
Primary human T cells isolated from PBMC were cultured in hTC Culture Medium supplemented with 10% FCS. 24h and 48h post plating cell viability was assessed by flow cytometry after staining cells with propidium iodide.
hTC Culture Medium provides ideal conditions for activation experiments.
Human T cells transfected with 2 µg pmaxGFP were activated with anti-CD3/anti-CD28 5h post nucleofection. 48h post nucleofection activation was determined by expression of IL-2Rα chain (CD25). The hTC Culture Medium shows a 20% increased activation compared to competitor medium optimized for lymphocyte culture.
