Citation Details
ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation
| Authors |  Faure S, Salazar-Fontana LI, Semichon M, Tybulewicz VL, Bismuth G, Trautmann A, Germain RN, Delon J |
| In | Nat Immunol (2004) 5(3): 272-279 |
| Cells used in publication | T cell, unstim., human Human unstimulated T cells Blood/Immune Cells Primary Cells Species: human Tissue Origin: blood |
| Substrate | Plasmid (general) |
| Topics | Cell morphology/cytoskeletonExpression of dominant-negative proteinMicroscopy of nucleofected cells |
| Research Area | Immunology / Hematology |
Research Field
A prerequisite for T cell activation is an intimate membrane contact with antigen-presenting cells (APCs), known as the immunological synapse. Here, the small GTPases Rac1, RhoA and Cdc42 are examined for their regulatory effect on ERZ (ezrin, radixin, moesin) proteins as molecular mediators of disanchoring of the cortical actin cytoskeleton from the plasma membrane, leading to a more efficient T cell-APC conjugate formation.
Nucleofection Experiments
Primary human peripheral blood T cells (PBTs) were nucleofected with expression vectors for GFP-tagged RhoA, Rac1, and Cdc42, as well as with expression vectors for mutated or dominant negative forms of these proteins.
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