Citation Details
Mammalian PAR-1 determines epithelial lumen polarity by organizing the microtubule cytoskeleton
| Authors |  Cohen D, Brennwald PJ, Rodriguez-Boulan E, Musch A |
| In | J Cell Biol (2004) 164(5): 717-727 |
| Genes of Interest | EMK1 |
| Cells used in publication | MDCK Tet-Off (BD) Epithelial Cells Cell Lines Species: canine Tissue Origin: kidney |
| Substrate | Plasmid (general) |
| Substrate Description | siRNA expression plasmid |
| Application | RNAi |
| Topics | Cell morphology/cytoskeletonIntercellular interaction/communicationMicroscopy of nucleofected cells |
| Research Area | Dermatology/Tissue Engineering |
| Relevant Products |  Nucleofector Device Cell Line Optimization Nucleofector Kit |
| Vector Backbone | pSUPER |
Research Field
PAR-1 is involved in the organisation of the microtubule network inducing the formation of intercellular lumina. It is suspected to regulate the developmental branching decision between hepatic and columnar epithelial cells.
Nucleofection Experiments
Knockdown of EMK1, a mammalian Par-1 homologue, by RNAi in MDCK cells resulted in a loss of 60-70% of protein. The cells showed an unpolarized microtubule cytoskeleton and lacked the ability to built lumina at cell-cell contact sites.
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