Citation Details
Autophagy Is a Defense Mechanism Inhibiting BCG and Mycobacterium tuberculosis Survival in Infected Macrophages
| Authors |  Gutierrez MG, Master SS, Singh SB, Taylor GA, Colombo MI, Deretic V |
| In | Cell (2004) 119(6): 753-766 |
| Cells used in publication | RAW 264.7 Blood/Immune Cells Cell Lines Species: mouse Tissue Origin: blood |
| Related cells | RAW 264.7 (ATCC) |
| Substrate | Plasmid (general) |
| Topics | ApoptosisDiseases (e.g. HIV)Microscopy of nucleofected cellsSignal transduction |
| Research Area | Immunology / Hematology |
| Relevant Products |  Nucleofector Device |
Research Field
Autophagy represents an underappreciated innate defense mechanism for control of intracellular pathogens like Mycobacterium tuberculosis, a pathogen that persists within phagosomes in macrophages.
Nucleofection Experiments
Cells from the mouse macrophage-like cell line RAW264.7 were nucleofected with several different expression plasmids coding for EGFP-fusions of wild-type and mutant LC3 (an elongation factor in autophagosome formation), coding for a GFP fusion of LRG-47 (a downstream effector of Interferon IFN-gamma), or coding for a FLAG-tagged Beclin-1.
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